Monday, September 7, 2009


1. Transcribe CLIA

-Chemiluminescence Immunoassay (CLIA)

2. Transcribe RLU

- relative light units (RLU)

3-4. Give the advantages of CLIA over the conventional colorimetric method.
-economical alternative to conventional colorimetric methodologies, such as Enzyme-linked immunosorbent assays (ELISA).
-does not require long incubations or the addition of stopping reagents, as is the case in some colorimetric assays

5. What is the labeled antibody used?
- horseradish peroxidase

6. Why is there a need of washing the solution?
-After a 60 minutes incubation at room temperature, the wells are washed 5 times by wash solution to remove unbound T4 conjugate

7-9. What is the relationship of emitted light to the amount of enzyme present and to the amount of unlabelled T4 in the sample?

-The intensity of the emitting light is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T4 in the sample.

10. What is the possible result if you fail to wash the solution?
- The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings

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